The film begins with the Phoenix retelling the tales and hopes of Astro Boy. Many of the visual being directly taken from the 1980s TV series as it recaps some of his adventures. 
Following this recap, Astro Boy confronts Blue Bon and tries to convince him to change his mind about the humans. But Blue Bon misunderstands, assuming that if Astro Boy does not agree then he is an enemy. Blue Bon hates humans because they broke apart his family (Maria and Tonto). A battle begins between the two after Astro Boy fails to convince Blue Bon that they aren't each others' enemy. 
(Source: Astro Boy Wiki)

Creating a robotic boy in the image of his recently deceased son Tobio, Professor Tenma thought nothing could go wrong now that his beloved child had been "reborn"... that is, until he comes to realize that this invention could never replace his son. Before long, however, the robot is kidnapped and spirited away to a robot circus in America, where he is soon rescued by Dr. Ochanomizu, and taken into his care. Tobio is given the new name Tetsuwan Atom, and transforms into Japan's crime-fighting hero, although his anti-violent pacifist views and overall childlike naivety tend to clash with his battles often. 
(Source: Anime News Network)

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