Isana appears in episode 22, &quot;Shrine in the Sea.&quot; She is Mio's daughter... and her mother. As Mana, she willingly sacrificed her time to the mushi at the bottom of the sea and was reborn as her daughter's daughter.

Neither good nor evil, they are life in its purest form. Vulgar and strange, they have inspired fear in humans since the dawn of time and have, over the ages, come to be known as "mushi." The stories of the Mushi and the people they affect are all linked together by a traveling Mushi-shi, or "Mushi Master," who seeks rare Mushi sightings and uses his shaman-like knowledge of Mushi to help the affected people.<br>
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What are the Mushi and what do they want?<br>
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(Source: Funimation)

Born in: Argyle, Texas, U.S.A.

She announced her retirement from voice acting due to difficulties recording at her home.

Non-anime roles:
~!Hermia in "Midsummer Night’s Dream" (play)  Juliet in "Romeo and Juliet Lead" (play)  Lisa in "Key Exchange" (play)  Magician's Assistant in "Norma's Place" (TV)  Principal/Dory in "Walker Texas Ranger" (TV)  Tiffany in "Invasion of the Baud iSnatchers" (play)!~

Tatiane "Tati" Keplmair Dias is a Brazilian actress, voice actor and dubbing director, best known for voicing Sakura Haruno in Naruto, Rukia Kuchiki in Bleach and Nami in One Piece. She is the older sister of [Thiago Keplmair](https://anilist.co/staff/100133/)